全文获取类型
收费全文 | 82310篇 |
免费 | 4008篇 |
国内免费 | 7245篇 |
出版年
2023年 | 785篇 |
2022年 | 976篇 |
2021年 | 1473篇 |
2020年 | 1617篇 |
2019年 | 2932篇 |
2018年 | 1935篇 |
2017年 | 1621篇 |
2016年 | 2031篇 |
2015年 | 3156篇 |
2014年 | 4232篇 |
2013年 | 5762篇 |
2012年 | 3627篇 |
2011年 | 4948篇 |
2010年 | 3589篇 |
2009年 | 3721篇 |
2008年 | 3972篇 |
2007年 | 4216篇 |
2006年 | 3830篇 |
2005年 | 3344篇 |
2004年 | 2766篇 |
2003年 | 2475篇 |
2002年 | 2140篇 |
2001年 | 1732篇 |
2000年 | 1541篇 |
1999年 | 1508篇 |
1998年 | 1371篇 |
1997年 | 1180篇 |
1996年 | 1099篇 |
1995年 | 1336篇 |
1994年 | 1245篇 |
1993年 | 1216篇 |
1992年 | 1219篇 |
1991年 | 1006篇 |
1990年 | 931篇 |
1989年 | 882篇 |
1988年 | 868篇 |
1987年 | 846篇 |
1986年 | 569篇 |
1985年 | 1003篇 |
1984年 | 1385篇 |
1983年 | 983篇 |
1982年 | 1342篇 |
1981年 | 947篇 |
1980年 | 960篇 |
1979年 | 909篇 |
1978年 | 532篇 |
1977年 | 428篇 |
1976年 | 350篇 |
1975年 | 271篇 |
1973年 | 276篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
91.
92.
93.
At room temperature, the chlorophyll (Chl) a fluorescence induction (FI) kinetics of plants, algae and cyanobacteria go through two maxima, P at ∼ 0.2-1 and M at ∼ 100-500 s, with a minimum S at ∼ 2-10 s in between. Thus, the whole FI kinetic pattern comprises a fast OPS transient (with O denoting origin) and a slower SMT transient (with T denoting terminal state). Here, we examined the phenomenology and the etiology of the SMT transient of the phycobilisome (PBS)-containing cyanobacterium Synechococcus sp PCC 7942 by modifying PBS → Photosystem (PS) II excitation transfer indirectly, either by blocking or by maximizing the PBS → PS I excitation transfer. Blocking the PBS → PS I excitation transfer route with N-ethyl-maleimide [NEM; A. N. Glazer, Y. Gindt, C. F. Chan, and K.Sauer, Photosynth. Research 40 (1994) 167-173] increases both the PBS excitation share of PS II and Chl a fluorescence. Maximizing it, on the other hand, by suspending cyanobactrial cells in hyper-osmotic media [G. C. Papageorgiou, A. Alygizaki-Zorba, Biochim. Biophys. Acta 1335 (1997) 1-4] diminishes both the PBS excitation share of PS II and Chl a fluorescence. Here, we show for the first time that, in either case, the slow SMT transient of FI disappears and is replaced by continuous P → T fluorescence decay, reminiscent of the typical P → T fluorescence decay of higher plants and algae. A similar P → T decay was also displayed by DCMU-treated Synechococcus cells at 2 °C. To interpret this phenomenology, we assume that after dark adaptation cyanobacteria exist in a low fluorescence state (state 2) and transit to a high fluorescence state (state 1) when, upon light acclimation, PS I is forced to run faster than PS II. In these organisms, a state 2 → 1 fluorescence increase plus electron transport-dependent dequenching processes dominate the SM rise and maximal fluorescence output is at M which lies above the P maximum of the fast FI transient. In contrast, dark-adapted plants and algae exist in state 1 and upon illumination they display an extended P → T decay that sometimes is interrupted by a shallow SMT transient, with M below P. This decay is dominated by a state 1 → 2 fluorescence lowering, as well as by electron transport-dependent quenching processes. When the regulation of the PBS → PS I electronic excitation transfer is eliminated (as for example in hyper-osmotic suspensions, after NEM treatment and at low temperature), the FI pattern of Synechococcus becomes plant-like. 相似文献
94.
95.
In experimentally infected insects, the sex ratio of first generation nematodes of five species of Steinernema was female-biased (male proportion 0.35-0.47). There was a similar female bias when the worms developed in vitro (0.37-0.44), indicating that the bias in these species is not due to a lower rate of infection by male infective juveniles (IJs). Experimental conditions influenced the proportion of males establishing in insects, indicating that male and female IJs differ in their behaviour. However, there was no evidence that males are the colonising sex in any species, contrary to what has previously been proposed. Time of emergence from the host in which the nematodes had developed influenced sex ratios in experimental infections. In three species (Steinernema longicaudum, Steinernema glaseri and Steinernema kraussei), early emerged nematodes had a higher proportion of males than those that emerged later, with the reverse trend for Steinernema carpocapsae and Steinernema feltiae. In a more detailed in vitro study of S. longicaudum, the proportion of males was similar whether or not the nematodes passed through the developmentally arrested IJ stage, indicating that the female bias is not due to failure of males to exit this stage. The sex ratio in vitro was independent of survival rate from juvenile to adult, and was female-biased even when all juveniles developed, indicating that the bias is not explained by failure of males to develop to adults. The female-biased sex ratio characteristic of Steinernema populations appears to be present from at least the early juvenile stage. We hypothesise that the observed female bias is the population optimal sex ratio, a response to cycles of local mate competition experienced by nematodes reproducing within insect hosts interspersed with periods of outbreeding with less closely related worms following dispersal. 相似文献
96.
Sotiris K. Hadjikakou Constantinos D. Antoniadis Maciej Kubicki Spyros Karkabounas 《Inorganica chimica acta》2005,358(10):2861-2866
A novel water stable, antimony(III) complex with the heterocyclic thioamide; 2-mercapto-pyrimidine (pmtH), of formula [Sb(pmt)3] · 0.5(CH3OH), has been synthesized and characterized by elemental analysis, 1H, 13C NMR and FT-IR spectroscopic techniques. Crystal structure of the molecule has been determined by X-ray diffraction at ambient conditions. The compound [C12H9N6S3Sb · 0.5(CH3OH)] is monoclinic, space group P21/c, a = 7.0646(7), b = 16.3767(14), c = 14.7265(13) Å, β = 92.016(7)°, Z = 4. In complex, three sulfur and three nitrogen atoms from thione ligands form a distorted pendagonal pyramidal geometry around antimony(III). The toxicity of the compound against tumor pleiomorphic cells, which has been isolated from a leiomyosarcoma tumor in the Wistar rat (chemical carcinogenesis using BaP) was studied in vitro. The results show that the compound did not destroy or prevent multiplication in vitro in leiomyosarcoma cells in low doses. The influence of the compound in the platelet aggregation, which correlates with the above tumor cells enhanced metastatic potential, has also been studied. The anti-metastatic capability study shows that the compound inhibited cancer cell induced aggregation up to the value of 10% in all mM concentrations tested. 相似文献
97.
The urticating setae of Ochrogaster lunifer,an Australian processionary caterpillar of veterinary importance 下载免费PDF全文
L. E. PERKINS M. P. ZALUCKI N. R. PERKINS A. J. CAWDELL‐SMITH K. H. TODHUNTER W. L. BRYDEN B. W. CRIBB 《Medical and veterinary entomology》2016,30(2):241-245
The bag‐shelter moth, Ochrogaster lunifer Herrich‐Schaffer (Lepidoptera: Notodontidae), is associated with a condition called equine amnionitis and fetal loss (EAFL) on horse farms in Australia. Setal fragments from O. lunifer larvae have been identified in the placentas of experimentally aborted fetuses and their dams, and in clinical abortions. The gregarious larvae build silken nests in which large numbers cohabit over spring, summer and autumn. The final instars disperse to pupation sites in the ground where they overwinter. Field‐collected O. lunifer larvae, their nests and nearby soil were examined using light and electron microscopy to identify setae likely to cause EAFL and to determine where and how many were present. Microtrichia, barbed hairs and true setae were found on the exoskeletons of the larvae. True setae matching the majority of setal fragments described from equine tissue were found on third to eighth instar larvae or exuviae. The number of true setae increased with the age of the larva; eighth instars carried around 2.0–2.5 million true setae. The exuvia of the pre‐pupal instar was incorporated into the pupal chamber. The major sources of setae are likely to be nests, dispersing pre‐pupal larvae and their exuviae, and pupal chambers. 相似文献
98.
Canopy closure estimation in a temperate forest using airborne LiDAR and LANDSAT ETM+ data 下载免费PDF全文
《植物生态学报》2016,40(2):102
Aims Forest canopy closure is one of the essential factors in forest survey, and plays an important role in forest ecosystem management. It is of great significance to study how to apply LiDAR (light detection and ranging) data efficiently in remote sensing estimation of forest canopy closure. LiDAR can be used to obtain data fast and accurately and therefore be used as training and validation data to estimate forest canopy closure in large spatial scale. It can compensate for the insufficiency (e.g. labor-intensive, time-consuming) of conventional ground survey, and provide foundations to forest inventory.Methods In this study, we estimated canopy closure of a temperate forest in Genhe forest of Da Hinggan Ling area, Nei Mongol, China, using LiDAR and LANDSAT ETM+ data. Firstly, we calculated the canopy closure from ALS (Airborne Laser Scanning) high density point cloud data. Then, the estimated canopy closure from ALS data was used as training and validation data to modeling and inversion from eight vegetation indices computed from LANDSAT ETM+ data. Three approaches, multi-variable stepwise regression (MSR), random forest (RF) and Cubist, were developed and tested to estimate canopy closure from these vegetation indices, respectively.Important findings The validation results showed that the Cubist model yielded the highest accuracy compared to the other two models (determination coefficient (R2) = 0.722, root mean square error (RMSE) = 0.126, relative root mean square error (rRMSE) = 0.209, estimation accuracy (EA) = 79.883%). The combination of LiDAR data and LANDSAT ETM+ showed great potential to accurately estimate the canopy closure of the temperate forest. However, the model prediction capability needs to be further improved in order to be applied in larger spatial scale. More independent variables from other remotely sensed datasets, e.g. topographic data, texture information from high-resolution imagery, should be added into the model. These variables can help to reduce the influence of optical image, vegetation indices, terrain and shadow and so on. Moreover, the accuracy of the LiDAR-derived canopy closure needs to be further validated in future studies. 相似文献
99.
100.
Soil is a non-renewable resource and its preservation is essential for food security, ecosystem services and our sustainable future. Simultaneously, it is a major challenge to substitute non-renewable fossil based resources with renewable resources to reduce environmental load. In order to check soil erosion vis-a-vis degradation of sloppy lands of rainfed maize–wheat rotation system, fertilization with organic manure supplemented with inorganic fertilizers is required. In order to address these issues, substitution of 50% NPK through four organic manures viz. farmyard manure (FYM), vermicompost (VC), poultry manure (PM) and in situ green manuring (GM) of sunnhemp (Crotalaria juncea L.) were evaluated against 100% NPK through inorganic fertilizers and through FYM for energy budgeting and emergy synthesis during 2009–2014. Integrated use of FYM along with 50% NPK fertilizers could maintain the highest energy ratio (7.3), human energy profitability (142.4), energy productivity (0.22 kg MJ−1), and energy profitability (6.3 MJ ha−1) over other treatments. However, GM and inorganic fertilizers on equal NPK basis maintained the highest energy intensiveness (24.61 MJ US $−1) and exhibited higher emergy yield ratio (2.66) and lower emergy investment ratio (0.60) and environmental loading ratio (3.74) which resulted into higher environmental sustainability index (0.71) over other treatments. Fertilization with organic manure (FYM) alone could not compete with other fertilized options to energy budgeting and emergy synthesis except specific energy. The study demonstrated that innovative integrated nutrient management of chemical fertilizers and organic manures particularly FYM for energy budgeting and GM for emergy synthesis may be considered as feasible and environment-friendly options for soil conservation, thereby benefiting a 50% saving on costly chemical fertilizers in non-OPEC countries which import most of its phosphorus and potassium fertilizers. 相似文献